Journal: Clinical immunology (Orlando, Fla.)

Article Title: Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells

doi: 10.1016/j.clim.2013.06.005

Figure Lengend Snippet: (A) CD1d expression in four medulloblastoma cell lines was examined by flow cytometry: staining with isotype control (tinted) or anti-hCD1d (open). (B) CD1d mRNA expression in four medulloblastoma cell lines was analyzed by RT-PCR using GADPH as an amplification control. (C) CD1d expression in primary tumors was assessed by IHC staining. Representative sections shown are from two CD1d-positive (upper pannel) and two CD1d-negative (lower pannel) of 20 analyzed tumors. Original magnification, X10. (D) CD1d mRNA expression was analyzed in 61 primary tumors by Taqman qRT-PCR and quantified using delta Ct method relative to the expression of HPRT1. DAOY and CHLA-255 cells were used as CD1d positive and negative controls, respectively.

Article Snippet: Immunohistochemistry Human medulloblastoma tissue array slides and normal brain paraffin tissue sections were purchased from Cybrdi, Inc. H&E staining was done following a standard hematoxylin and eosin staining protocol.

Techniques: Expressing, Flow Cytometry, Staining, Control, Reverse Transcription Polymerase Chain Reaction, Amplification, Immunohistochemistry, Quantitative RT-PCR

Journal: Advanced Science

Article Title: Tissue Mechanics and Hedgehog Signaling Crosstalk as a Key Epithelial–Stromal Interplay in Cancer Development

doi: 10.1002/advs.202400063

Figure Lengend Snippet: Nuclear GLI2 expression is closely associated with collagen accumulation in patients with OSCC. A) Representative images of H&E staining provided by TissueArray.Com. and IHC for GLI2 in tissue microarrays of human normal oral mucosal and OSCC tissues. B) The IHC scores for nuclear GLI2 expression, ranging from 0 to 9, for normal ( n = 33) and tumor ( n = 260) tissues. The mean mean ± s.e.m. results are displayed, and the Mann‐Whitney U test was conducted for statistics (**** p < 0.0001). C) The proportions of tissue microarray cases grouped by low (≤ 4) or high (> 4) GLI2 expression in normal and tumor tissues. A table indicates the number of cases in each group. Chi‐square test was used for statistics (**** p < 0.0001). D) ROC curves and the area under curve (AUC) value of high nuclear GLI2 expression for distinguishing OSCC tumors from normal tissues. E) Representative images of IHC for GLI2 or SR staining in serial sections of tissue microarrays of human OSCC tissues, showcasing varying GLI2 intensity scores (ranging from 0 to 3) and SR scores (ranging from 1 to 3). F) The IHC scores for nuclear GLI2 (ranging from 0 to 9) for OSCC tumor tissues with low SR scores (SR_low, ≤ 1) or high SR scores (SR_high, > 1). The mean ± s.e.m. results are displayed, and the Mann‐Whitney U test was performed (* p < 0.05). G) The proportions of tissue microarray cases grouped based on low (≤ 4) or high (> 4) GLI2 expression in cases with low SR scores (SR_low, ≤ 1) or high SR scores (SR_high, > 1). A table indicates the number of cases in each group. Chi‐square test was conducted (** p = 0.003). (H) The AUC of the ROC of high SR score in distinguishing OSCC tumors with high GLI2 expression from tumors with low GLI2 expression. Scale bars indicate the specified length (µm) under the bars.

Article Snippet: Normal and OSCC TMAs (OR208a, OR481a, OR601c) were purchased from TissueArray.Com (USA).

Techniques: Expressing, Staining, MANN-WHITNEY, Microarray